SKi Pro is the name of a line of modules which together form a complete, automated system for studying biomolecular interactions without labels. Most often the modules are used together to form an integrated instrument, though the modules can be purchased and used separately. Full lists of specifications and applications are available. Quotes are available for any configuration.
The X10 is a fully parallel, dual channel flow system for studying biomolecular interactions. Each channel is controlled by its own independent, continuous pumps from the SKi AutoFlow. The AutoPrep, autosampler gives fully unattended use with two standard well plates, most commonly configured with a 96 well plate (deep or shallow) for samples and a 12, 10 mL vial well plate for commonly used buffers. The system is capable of studying many types of biomolecular interactions including antibody binding, protein-protein interactions and protein-small molecule interactions. Combined with your existing mass spectrometer, SKi Pro opens up one-of-a-kind, fully on-line molecular interation kinetics-mass spectrometry applications (MIK-MS).
The AutoFlow module drives the fluidics of SKi Pro. This module, which is often purchased as a stand-alone module to perform e.g. off-line fluidics experiments, contains high quality liquid chromatography components connected by commodity tubing. This allows users to easily replace tubing as necessary, obviating the need for a service engineer for routine maintenance. The system uses in-line vacuum degassing and can switch between six different buffers.
The AutoPrep is the autosampler in SKi Pro. This module contains space for two SBS trays and is usually configured with a 96 well plate and a 12 vial plate, containing the buffers for the system. The AutoPrep is fully integrated into SKi Pro to deliver samples to either the sample or reference loops, and may be used in stand alone mode to deliver samples to up to six different channels. Autosampler washing is integrated into the unit which features a metal-free path for all samples and cooling down to 4°C.
The world of label free binding uses aqueous buffers at ambient pressures. The world of mass spec uses mixed aqueous-organic buffers at high — sometimes very high — pressures. To bridge those worlds we offer SKi Bridge, containing two ultra-high pressure, 10-port, 2-position valves and the necessary electronic interface for SKi Pro to bridge to your existing mass spectrometer. SKi Bridge may also be used as a stand-alone, fully computer controlled module for general interfacing needs.
The control software which comes with SKi Pro runs on any standard Windows laptop and is called SKi Report. It has four separate components.
Data acquisition methods may be programmed graphically using either wizards or a low level interface which sets the full system state at controllable points in time.
Instrument setup allows the user, when necessary, to view the logs and set low level parameters which affect control of the instrument.
Data acquisition takes place in real time with a graphical readout of all of the components in the system.
Data analysis implements a full non-linear least squares fitting algorithm that allows for fitting of single traces, multiplied traces, or even multiple traces from multiple files, singly or globally with a variety of different models. Analyzed data may be exported, or copy and pasted, from the program into any standard Windows program. Analysis methods may also be programmed to automatically apply a specific analysis to a data set once complete. Data analysis may also be carried out offline when not connected to the instrument.