3D Biomolecular Interaction Analysis


Product Specifications

Below you will find the low level specifications of the SKi Pro X10 and the three modules which can be purchased separately. Ordering information may be obtained by requesting a quote online or contacting Silicon Kinetics.

SKi Pro X10

Summary:Integrated instrument for automated, label-free biomolecular interaction analysis
Detection Technology:Nanopore Optical Interferoemtry
Biosensor Type:PEG-coated, porous silicon
Available surface chemistries:Carboxyl, benzaldehyde, NTA, SA, PrA, PrG
Information Provided:Time-resolved binding and dissociation
Analysis time per Cycle:10 minutes (typ.)
Automation:Autosampler and scheduled injections
Sample Types:Protein, nucleic acids, small molecules, biological fluids
Buffers:Aqueous buffers (pH 0–9), up to 5% DMSO
Sample Volume:90 µL (typ)
Sample Concentration:100 pg/mL to 5 mg/mL
Injection Volume:50 µL (typ)
Flow Rate:1–200 µL/min
Flow Cell Volume:400 nL
Flow Cell Dimensions:0.16 x 1.0 x 2.4 mm
Sample Capacity:2 SBS Well Plates (12 & 48 vial, 96 & 384 well)
Analysis Temperature:10°C below ambient to 60°C
Sample Storage Temperature:4°C to ambient
Buffer Capacity:up to 6
Referencing:parallel, on cartridge
Number of Flow Cells:2
Included:AutoPrep, AutoFlow, SKi Report, power cord, ethernet cable, fluidics kit, buffer tray with bottles, carboxyl coupling kit, COOH SKi Sensors, Windows laptop
Use:Indoor use only
Working Range
Association rate (kon):103–106 M-1sec-1
Dissociation rate (koff):0.1-106 sec-1
Molecular Mass Limit of Detection:100 Da
Quantification:typically 0.01–100 µg/mL with antibody capture
Baseline Noise (RMS):<0.01 nm
Baseline Drift:<0.2 nm/h
Mass Spectrometer Integration:In-line, SKi Bridge or Fraction Collection
External Dimensions:16" x 36" x 23" (H x W x D)
Weight:68 lbs (excludes AutoPrep and buffer tray)
Power Requirements:100-240V 50-60 Hz}
Analyzer Power:3A at 100 VAC (max)
Autoprep Power:2A at 100 VAC (max)
Safety Standard:CE
Data Handling
Operating System:≥Windows 8
Software:SKi Report
Data Presentation:.jpg, .emf saving & text export
Data Export Formats:clipboard or file
Data Connection:ethernet (RJ-45)

SKi AutoFlow

Summary:Standalone, or integrated autosampler for SKi Pro
Operation:SKi Report Integration or Windows Operation
SKi Report Integration:Fully method programmable
Windows Operation:Ethernet connection (RJ-45), fully programmable
Included:Power supply, ethernet cable, fluidics kit, SKi Report software
Use:Indoor use only
Dimensions:16" x 8" x 11" (H x W x D)
Weight:25 lbs
Power requirements:24V DC, 3A (PS included)
Injection Valves:2, 2 position 6-port valves
Injection Valve Ports:M4 threads, 1/32" tubing OD
Injection Valve port-port volume:80 nL
Injection Valve pressure limit:5,000 psi (340 bar)
Fluid Path:Metal-free and biocompatible
Selection Valve:6 position 7-port valve
Selection Valve Ports:1/16" tubing OD, ferrule fit
Selection Valve port-port volume:~15 µL
Selection Valve pressure limit:100 psi (7 bar)
Pumps:2-continuous, self-priming, bidirectional
Pump pressure limit:100 psi (7 bar)
Pump flow rate:1-200 µL/min
Vacuum Degasser:in-line
Degassing chambers:5, teflon
Degassing operating range:<80 torr
Pressure relief valves:2, 100 psi
Master connectors:2 USB Type A, generally controls AutoPrep and SKi Bridge

SKi AutoPrep

Summary:Standalone, or integrated, low pressure fluidics unit
Operation:SKi Report Integration or Windows Operation
Injection modes:Full-loop, Partial loop-fill, µL-pick-up
Injection volume:1–250 µL, (90 µL typical)
Injection precision:<0.3%, typical
Sample viscosity:0.1–5 cP
Needle wash:on board wash, inside, outside, air dry
Injection cycle time:<60 seconds
Valve switching time:60 msec
Fluid Path:PEEK & silica coated steel
Sample capacity:2 SBS Well Plates (12 & 48 vial, 96 & 384 well)
Maximum vial height:47 mm
Sample cooling:Ambient to 4°C±2°C
Included:USB cable, power cord, waste tubing, wash buffer container, 12-vial holder, 48-vial holder, cooling insulation
Use:Indoor use only
Dimensions:12" x 23" x 14" (H x W x D)
Weight:42 lbs
Max load on top cover:143 lbs
Power requirements:95–240 Volt AC ± 10%; 50–60 Hz; 200 VA
Communication:USB 2.0, type B connector

SKi Bridge

Summary:Standalone, or integrated interface between SKi Pro and LC-MS systems
Operation:SKi Report Integration, Windows Operation, Direct Control or Stand Alone
SKi Report Integration:Fully method programmable
Windows Operation:USB 2.0 with utility program
Direct Control:Digital signals control valve positions, H/L on input pins
Stand Alone:key pad control with position readout
Included:USB cable, power supply, three spools 16" tubing, I/O green block, 20 medium pressure fittings, 20 ultra-high pressure fittings
Use:Indoor use only
Valves:2, 2-position 10 port valves
Fluid path:Coated SS Stators, PEEK based rotors
Valve ports:10–32 threads, 1/16" tubing OD
Port‐port‐volume:0.14 µL
Port diameter:150 µm
Max Pressure:15,000 psi (1,030 bar)
Electrical Interface:2 programmable DIOs, contact closure out, optocoupler input, 5V out, gnd
DIOs:5V, diode protected
Contact closure out:programmable time
Optocoupler in:3–24 V
5V out:40 mA
Dimensions (HxWxD):6" x 7" x 12" (15 x 18 x 30 cm)
Weight:6 lbs (3 kg)
Electrical:100–240V, 50–60Hz, ~1.5A
Communication:USB 2.0, type B connector